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Visiting Professor του ΔΠΜΣ “Έρευνα στη Γυναικεία Αναπαραγωγή”

Ανακοινώσεις

Με μεγάλη χαρά σας ανακοινώνουμε ότι ο επί πολλών ετών Visiting Professor του ΔΠΜΣ “Έρευνα στη Γυναικεία Αναπαραγωγή”
Καθηγητής Ενδοκρινολογίας του Πανεπιστημίου του Βελιγραδίου κ. Djuro Macut ορίστηκε εντολοδόχος Πρωθυπουργός της Σερβίας και καλείται σύντομα να σχηματίσει τη νέα κυβέρνηση.

Ο Καθηγητής κ. Djuro Macut, στενότατος συνεργάτης πολλών ετών και εγκάρδιος φίλος του Διευθυντή του ΔΠΜΣ “Έρευνα στη Γυναικεία Αναπαραγωγή” Καθηγητή κ. Γεωργίου Μαστοράκου τελεί χρέη Επισκέπτη Καθηγητή τόσο στην Μονάδα Ενδοκρινολογίας Σακχαρώδη Διαβήτη και Μεταβολισμού στο Αρεταίειο Νοσοκομείο, υπό την υπευθυνότητα του Καθηγητού κ. Γ. Μαστοράκου, ενώ παράλληλα ασκεί διδακτικό και ερευνητικό έργο και είναι υπεύθυνος για το συντονισμό και την επίβλεψη διπλωματικών εργασιών μεταπτυχιακών φοιτητών του ΠΜΣ, με στόχο τη δημοσίευσή τους σε διακεκριμένα διεθνή περιοδικά.

Ο Διευθυντής του ΔΠΜΣ “Έρευνα στη Γυναικεία Αναπαραγωγή” Καθηγητής κ. Γεώργιος Μαστοράκος, η Επιτροπή Προγράμματος Σπουδών, η γραμματέας και οι φοιτητές ευχόμαστε ολόψυχα στον Καθηγητή κ Macut ένα λαμπρό έργο αντάξιο του ήθους που τον διακρίνει.

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The Role of Lipid Accumulation Product (LAP) as a Metabolic Indicator in Polycystic Ovary Syndrome (PCOS)

Δημοσιευμένες εργασίες

Ioannis Prokopakis, Vasilios Pergialiotis, Ioakeim Sapantzoglou, Eleftherios Zachariou, Antonios Koutras, Nikolaos Thomakos, Kalliopi Pappa

Polycystic ovary syndrome (PCOS) presents one of the most widespread endocrine disorders affecting women of reproductive age, predominantly characterized by hyperandrogenism, anovulation, and polycystic ovaries. Women diagnosed with PCOS also present an increased risk for developing metabolic complications like dyslipidemia, metabolic syndrome (MetS) insulin resistance (IR) and type 2 diabetes mellitus. With effective early detection becoming critical to managing these adverse effects, Lipid Accumulation Product (LAP) has emerged as a reliable marker for detecting excessive lipid accumulation beyond adipose tissue, such as in the liver, often associated with cardiometabolic risk; especially among patients with PCOS. This review examines the link between the application of LAP as a predictor of cardiometabolic risks and its association to PCOS, hence seeking possible implementations in clinical settings towards early diagnosis and improved management for affected individuals.

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Hofbauer cells morphology and density in placentas from normal and pathological gestations

Δημοσιευμένες εργασίες

Charalampos Grigoriadis, Aliki Tympa, Maria Creatsa, Panagiotis Bakas, Angelos Liapis, Agathi Kondi-Pafiti, Georgios Creatsas

Abstract
PURPOSE: In placentas from uncomplicated pregnancies, Hofbauer cells either disappear or become scanty after the fourth to fifth month of gestation. lmmunohistochemistry though, reveals that a high percentage of stromal cells belong 1o Hofbauer cells. The aim of this study was to investigate the changes in morphology and density of Hofbauer cells in placentas from normal and pathological pregnancies. METHODS: Seventy placentas were examined: 16 specimens from normal term pregnancies, 10 from first trimester’s miscarriages, 26 from cases diagnosed with chromosomal abnormality of the fetus, and placental tissue specimens complicated with intrauterine growth restriction (eight] or gestational diabetes mellitus !1 OJ. A histological study of hematoxylin-eosin (HE) sections was performed and immunohistachemical study was performed using the markers: CD 68, Lysazyme, Al Antichymotrypsine, CK-7, vimentin, and Ki-67. RESULTS: In normal term pregnancies, HE study revealed Hofbauer cells in 37.5% of cases while immunohistochemistry revealed in 87.5% of cases. In first trimester’s miscarriages and in cases with prenatal diagnosis of fetal chromosomal abnormalities, both basic and immunohistochemical study were positive for Hofbauer cells. In pregnancies complicated with intrauterine growth restriction or gestationa I diabetes mellitus, a positive immunoreaction was observed in 100 and 70% of cases, respectively. CONCLUSIONS: Hofbauer cells are present in placental villi during pregnancy, but with progressively reducing density. The most specific mark.er for their detection seems to be A 1 Antichymotrypsine. It is remark.able that no mitotic activity of Hofbauer cells was noticed in our study, as the mark.er of cellular multiplication Ki-67 was negative in all examined specimens.

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Assessing the practice of LuPOR for poor responders: a prospective study evaluating follicular fluid cfDNA levels during natural IVF cycles

Δημοσιευμένες εργασίες

Sfakianoudis Konstantinos, Tsioulou Petroula, Maziotis Evangelos, Giannelou Polina, Glava Argyro, Grigoriadis Sokratis, Rapani Anna, Nezos Andrianos, Pantou Agni, Koutsilieris Michael, Pantos Konstantinos, Mastorakos George, Simopoulou Mara

Abstract
Purpose The aim of this study is to provide data on the practice of Luteal Phase Oocyte Retrieval (LuPOR). The authors assess cell-free DNA levels in follicular fluid (ff cfDNA) from poor responders undergoing natural cycles, and comparing it to respective data originating from follicular phase oocyte retrievals.
Methods Forty-seven women were eligible for this prospective study. Participants were classified as poor responders based on Bologna criteria while being detected with a second follicular wave. Follicular fluid was collected and prepared for cfDNA extraction. Levels of cfDNA were quantified via Q-PCR employing the ALU115 and ALU247 primers. These primers are associated with apoptotic and necrotic events. Levels of ff cfDNA resulting from follicular phase oocyte retrieval (FoPOR) and LuPOR-performed in a single menstrual cycle were associated with the number and maturation status of yielded oocytes and the number and fertilization status of resulting zygotes. Survival rate following thawing of cryopreserved zygotes, along with the resulting number of cleavage stage and blastocyst stage embryos are provided.
Results Mean levels of ALU115 were significantly lower during FoPOR when compared to LuPOR (0.79 ± 0.72 vs 1.46 ± 1.59 ng/μl, p = 0.02). Regarding the FoPOR group, a significant positive correlation of serum estradiol and ALU115 concentration (p = 0.04) was revealed. A significant negative correlation between serum estradiol and cfDNA integrity was observed both during FoPOR (p = 0.03) and LuPOR (p = 0.03). A significant lower number of retrieved (1.09 ± 0.28 vs 1.29 ± 0.58, p =0.02) and MII oocytes (0.77 ± 0.55 vs 1.08 ± 0.61, p = 0.02) was observed when comparing the FoPOR to LuPOR groups respectively. The integrity of cfDNA was observed to be higher in FoPOR originating embryos that arrested either prior to cleavage (0.28 ± 0.13 vs 0.17 ± 0.10, p = 0.006) or prior to blastocyst formation (0.28 ± 0.12 vs 0.13 ± 0.06, p = 0.04). In the case of LuPOR originating embryos, cfDNA integrity was observed to be higher in embryos that arrested only prior to the blastocyst stage (0.27 ± 0.20 vs 0.11 ± 0.07, p = 0.008). Similarly, cfDNA integrity was observed to be lower in top quality blastocysts originating from FoPOR (0.07 ± 0.04 vs 0.17 ± 0.05, p = 0.03) and in top quality cleavage stage embryos (0.09 ± 0.06 vs 0.31 ± 0.22, p = 0.01) and blastocysts (0.06 ± 0.02 vs 0.14 ± 0.06, p = 0.02) originating from LuPOR.
Conclusions Our results indicate that ff originating from LuPOR presents with higher levels of cfDNA. The higher cfDNA levels are attributed to mainly apoptotic events, as the ALU247 levels and DNA integrity did not differ statistically significantly between FoPOR and LuPOR. The absolute mean level of ALU247 corresponding to necrotic events was higher in LuPOR. Regarding embryological data, cfDNA integrity was correlated with both number and quality of cleavage stage embryos in both FoPOR and LuPOR, along with blastocyst stage embryos in LuPOR. Necrotic events were associated with poorer blastocyst formation rate and blastocyst quality in LuPOR. As the comparison between FoPOR and LuPOR results to similar IVF laboratory data, the practice of LuPOR may stand as a promising approach for poor responders, while it merits further investigation.

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